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  • RL0001.jpg

6-FAM phosphoramidite 204697-37-0


Name


CAS


Catalog#


Formula


Molecular Weight


Appearance


Purity


Storage


6-FAM phosphoramidite

204697-37-0

RL0001

C46H58N3O10P

843.95

White solid

97%

-15℃ Avoid light and moisture

Classification

FAM

产品详情


Product Introduction

  The phosphamide technique is well suited to modify synthesized oligonucleotides with covalently linked reporter groups, linkers, spacers or haptens. 6-FAM, derived from the single isomer 6-carboxyl fluorescein, 5' -hexachlorofluorescein CE phosphamide (HEX) and 5' -tetrachlorofluorescein CE phosphamide (TET) can all be used to effectively label oligonucleotides.

  Labeling of the 3' end of an oligonucleotide with fluorescein can be achieved using 3' -fluorescein CPG. When any of these carriers are used, standard cleavage and deprotection of fluorescein labeled oligonucleotides are performed with ammonium hydroxide. Since fluorescein phosphoamides do not contain dmt groups, demethylation steps are not required. After ammonia evaporation, the labeled oligonucleotides can be used for most applications.

  Excitation wavelength (nm) : 496

  Emission wavelength (nm) : 520

  Changsha HanchBio has completed the KG process development of the product, and the product has been provided to a large number of domestic IVD enterprises, product quality is stable and reliable, the difference between batches is better than the imported products.